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发布于:2021-2-24 05:05:11  访问:116 次 回复:0 篇
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Th normal goat serum at room temperature forFrozen abdominal aorta tissues
Journal of Translational Medicine 2013, 11:308 http://www.translational-medicine.com/content/11/1/Page 4 ofwas performed with Lanabecestat Autophagy t-test and chi-square test. Table 1 showed biochemical alterations of the three groups of rats in chronic Toxoflavin MedChemExpress pubmed ID:https://www.ncbi.nlm.nih.gov/pubmed/6548916 study. Probe and primers were purchased from Taqman gene expression assays on demand (Applied Biosystems) for GAPDH (Rn99999916_s1), Runx2 (Rn01512299_m1), Osteocalcin (Rn00566386_g1), TRAP (Rn01752289_m1), Cath.k (Rn00580723_m1), RANKL (NM_057149.1) and OPG (NM_012870.2). GAPDH served as an internal control, and all results were expressed as the ratio of target RNA/GAPDH.Statistical analysisData were analyzed using statistical package SPSS IBM 20.0 and described as mean ?SD. Univariate analysisChe et al. Journal of Translational Medicine 2013, 11:308 http://www.translational-medicine.com/content/11/1/Page 4 ofwas performed with t-test and chi-square test. Comparison between different groups, analysis of variance was performed followed by one-way analysis of variance (ANOVA). Correlations were assessed by Pearson correlation test. If the data were not normally distributed, nonparametric tests were used (Mann hitney and Spearman correlation). p < 0.05 was considered significant.ResultsHampered hyperphosphatemia and varied osteoclastassociated hormones in serum after 2 La treatedabout nine times higher in group B (300.5 ?19.1 U/L) than in control group. 2 La treatment of CRF rats resulted in decreases in serum ALP activity (208.9 ?14.3 U/L, p < 0.01 vs group B) at 10th week. We also tested the role of circulating levels of RANKL and OPG as possible markers for vascular calcification in our model. OPG was dramatically elevated in group B (2722.4 ?153.5 U/L) and C (2584.1 ?147.8 U/L, p < 0.05 vs group B) at 10th week. Interestingly, the serum level of RANKL was also markedly elevated that RANKL/OPG ratio was not modified among the three groups (Table 2).The mortality was low during the whole study and only one rat was died at week 6 in 2 La group. Table 1 showed biochemical alterations of the three groups of rats in chronic PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/6548916 study. As expected, serum creatinine (Cr) was similarly increased in uremic rats compared with normal ones at week 4 (group B 352.7 ?31.6 mol/L and group C 347.3 ?27.3 mol/L respectively, p < 0.01). Serum phosphate was markedly increased in group B (6.94 ?0.97 mmol/L) and group C (5.12 ?0.84 mmol/L) at week 4, while the latter group diminished significantly PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/11139429 (2.92 ?0.73 mmol/L) compared with CRF group (3.48 ?0.69 mmol/L, p < 0.01) at the week 10, but still higher than normal rats (p < 0.01). Ten weeks of uremia increased serum PTH levels, while treatment with 2 La did not suppress this at 10th week (1643 ?115 mmol/L). Another parameter alkaline phosphatase (ALP) wasArterial medial calcification were mitigated along with bone-associated proteins altered via 2 La treatmentCalcification in great arteries was evaluated by VonKossa‘s and Masson‘s trichrome staining. The percentage of cross sections with mild, moderate-to-severe medial calcification (ie.
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