Nloaded and (b) Agloaded P(3HB)/P(Title Loaded From File 3HOco3HD). Finally, a suitable keratinocyte function was confirmed from the expression of cytokeratin at a protein degree soon after seven days in culture. These preliminary findings highlight the suitability of the created AgNPfunctionalized PHAbased nanofibers for biomedical applications, specifically as wound dressings.Supplementary Resources: The next are available online at https://www.mdpi.com/article/ ten.3390/ma14174907/s1, Figure S1: Fuel Chromatography Mass Spectrometry (GCMS) outcomes of (a) P(3HB), (b) P(3HOco3HD), Figure S2: 13 C Nuclear Magnetic Resonance (NMR) final results of (a) P(3HB), (b) P(3HOco3HD), Figure S3: FTIRATR spectrum of neat P(3HB)/P(3HOco3HD) nanofibers (black) and AgNPcontaining P(3HB)/P(3HOco3HD) nanofibers (red), Figure S4: SEM photographs of AgNPcontaining P(3HB)/P(3HOco3HD) nanofibers obtained by (a) Title Loaded From File protoco.Nloaded and (b) Agloaded P(3HB)/P(3HOco3HD). Cell nuclei are stained in blue. days in: (a) unloaded and (b) Agloaded P(3HB)/P(3HOco3HD). Cell nuclei are stained in blue. seven days in: (a) unloadedand (b) Agloaded P(3HB)/P(3HOco3HD). Cell nuclei are stained in blue. Scale bar is 50 . Unique magnification x10 Scale bar is 50 . Authentic magnification x10 Scale bar is 50 . Original magnification 0.4. Conclusions 4. Conclusions The aim of this research was to produce wound dressing prototypes from biobased The aim of this study was to produce wound dressing prototypes from biobased polymers, namely PHAs. Only quite thin layers of pure P(3HOco3HD) scaffolds might be polymers, namely PHAs. Only very thin layers of pure P(3HOco3HD) scaffolds could possibly be prepared in the ecofriendly solvent system, because the fibers were fused resulting from the lower Tg prepared from the ecofriendly solvent technique, because the fibers were fused as a result of the lower T prepared in the ecofriendly solvent system, as the fibers had been fused resulting from the low Tgg and elastomeric nature on the polymer. The morphology and elasticity of your nanofibers the polymer. The morphology and elasticity on the nanofibers and elastomeric nature of the polymer. The morphology and elasticity in the nanofibers had been optimized by blending P(3HOco3HD) with P(3HB), which features a greater Tgg but lacks were optimized by blending P(3HOco3HD) with P(3HB), which features a greater Tg but lacks optimized by blending P(3HOco3HD) with P(3HB), which includes a larger T but lacks have been ideal mechanical properties demanded for wound dressing applications due to its a lot more brittle nature. Given that P(3HB) had bad solubility in ecofriendly solvents, a chlorinated solventbased procedure consisting of chloroform and 2butanol was utilized for the electrospinning approach, and P(3HB)/P(3HOco3HD) nanofibers with an average diameter of 410 180 nm may very well be obtained. Afterwards, the surface of the fabricated P(3HB)/P(3HOco3HD) nanofibers was modified with AgNPs through a dipcoating system. The amount of AgNPs around the P(3HB)/P(3HOco3HD) nanofibers was determined as 18.05 wt and five.29 atomic by EDX characterization. Metabolic action, immunomodulatory, indirect antimicrobial action and cytokeratin expression of each the modified nanofibers wereMaterials 2021, 14,14 ofexamined in vitro working with HaCaT cells, revealing the possible ability to efficiently contribute to your wound healing method.