NRTI drug IC50s for primer grip mutants within the context of the TAM-containing polymerase domaind4T ABC Fold increasec Avasimibe Autophagy Indicate IC50 ( M) SE Fold boost 3TC Necessarily mean IC50 ( M) SE Fold increase ddI Mean IC50 ( M) SE PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/25799995?dopt=Abstract Fold increaseJ. Preceding facts ��-Tocopherol Metabolic Enzyme/Protease confirmed that TAMs raise the frequency of template switching PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/25766527?dopt=Abstract over WT (18). (B) The RT template-switching frequency for RNase H primer grip mutants while in the context of the TAM (T) polymerase area. Each and every bar signifies the RT template-switching frequency (% GFP reconstitution) mainly because it was measured from the single-cycle direct repeat deletion assay (suggest of two impartial experiments typical problems). Statistically sizeable variances ( ) in RT template switching ended up calculated by t take a look at (P 0.05 versus TAM manage) (dotted reference line). (C) Regression investigation. Boosts (n-fold) in AZT resistance ended up plotted vs . the RT template-switching frequency for RNase H primer grip mutants that contains a TAM polymerase domain.DELVIKS-FRANKENBERRY ET AL. Desk one. NRTI drug IC50s for primer grip mutants while in the context of the TAM-containing polymerase domaind4T ABC Fold increasec Mean IC50 ( M) SE Fold improve 3TC Necessarily mean IC50 ( M) SE Fold enhance ddI Indicate IC50 ( M) SE Fold increaseJ. VIROL.TDF Indicate IC50 ( M) SE Fold increaseMutationaMean IC50 ( M) SEbWT TAM G359A (T) A360K (T) H361A (T) K390A (T) K395A (T) E396A (T) T473M (T) Q475A (T) K476A (T) Y501A (T) I505A (T)a b1.5 two.6 2.six two.6 2.two one.nine 1.nine 1.4 two.four 2.eight three.7 two.one four.0.23 0.26 0.24 0.fifty six 0.24 0.77 0.33 0.45 0.ninety nine 0.15 1.01 0.fifty eight 1.one.0d 1.seven one.seven one.7 one.five 1.three 1.3 0.9 1.six one.9 2.5 one.4 two.5.one eight.8 eleven.4 seventeen.four nine.eight eleven.eight 11.two 13.one 9.five twelve.three sixteen.8 nine.3 7.0.forty one.00 0.25 one.28 one.33 0.63 0.99 three.01 0.sixty one.20 one.03 0.80 one.one.0d 1.seven two.two 3.4d one.nine two.3 2.2 two.6 1.9 two.four three.3d 1.8 one.six.fifty 18.eight fifteen.1 thirty.five 13.three 21.0 10.3 12.0 20.0 26.eight thirty.0 23.one.fifty four.seventeen 3.39 7.57 1.fifty six six.01 six.22 0.92 3.46 12.09 twelve.80 4.24 ND1.0 two.nine 2.three four.7 two.0 three.2 one.6 0.six one.8 four.1 4.6 3.19.two 17.0 19.eight twenty.3 fourteen.7 12.two 14.six 13.9 twenty five.seven PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/25843005?dopt=Abstract 35.8 fourteen.four thirteen.two 14.2.5 2.seven 0.four 0.9 1.one 1.3 0.7 7.0 five.8 three.3 three.nine 0.3 6.one.0 1.1 one.one 1.one 0.9 0.8 0.eight 0.eight one.8 two.5d 0.9 0.nine 1.two.4 2.4 three.0 2.eight one.five three.one 2.2 two.seven two.five 5.4 three.nine four.nine 2.0.22 0.16 0.sixteen 0.thirteen 0.ten 0.05 0.twenty 0.21 0.46 0.forty two 0.70 0.32 0.1.0 1.0 one.second 1.1 0.6d one.3d 0.nine one.1 one.0 2.3d 1.6d two.0d one.T, TAM that contains.